organic acids test (oat) + GPl-SNP1000 dna sequencing profile


The goal of every healthcare practitioner should be to provide their patients with the most personalized medicine possible.  This involves having customized diagnostics and treatment plans to best meet the needs of the individual patient and help the patient achieve better health outcomes.  The Organic Acids Test and the GPL-SNP1000 DNA Sequencing Profile from The Great Plains Laboratory, Inc. can work in tandem to achieve these outcomes.  The Organic Acids Test (OAT)  gives us a comprehensive metabolic snapshot of multiple pathways in the body, offering insight into possible underlying causes of symptoms, as well as what kind of nutritional support is needed.   It can be used along with GPL-SNP1000, which tells us what genetic mutations a patient has and what a patient’s predisposition is for having various disorders.  The primary pathways where we see overlap between the OAT and GPL-SNP1000 are methylation, mental health, detoxification, and oxalate metabolism.


Mental Health
The first pathway that is helpful to analyze in both the OAT and GPL-SNP1000 is the mental health pathway, which involves the synthesis and breakdown of neurotransmitters in the brain. The combination of measuring the neurotransmitter metabolites and knowing if the enzymes involved are functional will help guide us to the best treatment options. GPL-SNP1000 covers 14 diff erent mental health genes. Three of the best markers in the OAT for measuring neurotransmitter metabolism are homovanillic acid (a dopamine metabolite), vanilymandelic acid (epinephrine/norepinephrine), and 5-HIAA (serotonin). These markers are the metabolites of the neurotransmitters by the enzymes MAOA and COMT, the genes for which are analyzed in GPL-SNP1000. Defi ciencies in these enzymes due to faulty SNPs may cause low neurotransmitter levels, which may also be caused by low amounts of precursors, cofactors, and increased inhibitors.

The next pathway that GPL-SNP1000 covers is the DNA methylation pathway, also called the MTHFR pathway.  This process is responsible for the formation compounds that are used in neurotransmitter metabolism, detoxification, nucleotide synthesis, and multiple other important processes.  GPL-SNP1000 looks at 105 different methylation SNPs (single-nucleotide polymorphisms.  The first marker pertinent to methylation on the OAT is vitamin B12.  We evaluate B12 levels by measuring the amount of methylmalonic acid.  B12 is an important cofactor for many of these methylation enzymes.  The second important marker is pyridoxic acid, which is a form of vitamin B6, an important cofactor in the methylation pathway.  It is directly involved with the function of the CBS enzyme and indirectly involved with MTHFR, BHMT, and SHMT.  Another marker involved with the MTHFR pathway is uracil.  Having an elevated uracil level can be indicative of folate pathway malfunction. 

The third pathway to look at with both the OAT and GPL-SNP1000 is detoxification, and specifically for glutathione (GSH).  Detoxification is so important in today’s industrial, polluted, and toxic world.  Every day we are inundated by hundreds of chemicals.  We are exposed to many through the environment and some by choice (like medications).  Our bodies have to process these chemicals in some way.  GPL-SNP1000 looks at dozens of genes that are important for detoxification.  A good marker in the OAT for how well the body is detoxifying is pyroglutamic acid.  Elevated values of pyroglutamic acid are indicative of glutathione deficiency due to excessive toxic exposure or a genetic issue. 

Oxalate Metabolism
Oxalate metabolism is another important pathway to analyze. Oxalates are crystalline molecules that we absorb from our diet (high oxalate foods) or are produced by an infection, like yeast/fungal overgrowth, that accumulate in the body and cause infl ammation. The symptoms of oxalate accumulation include pain, nephrolithiasis, and neurological symptoms. Oxalates are known to cause/create kidney stones. Children with autism who exhibit eye-poking behavior have been shown to have a build-up of oxalates behind their eyes, causing tremendous pain, and thus the eye-poking. GPL-SNP1000 covers fi ve diff erent genes involved with the production and elimination of oxalates. The OAT has three oxalate markers: glyceric, glycolic, and oxalic acids. In addition, low B6 and increased yeast or fungal markers are associated with increased oxalates.


Organic Acids Test: 10 mL of first morning urine before food or drink is suggested. Patient should avoid apples, grapes (including raisins), pears, cranberries and their juices 24 hours prior to specimen collection.  Avoid arabinogalactan, echinacea, reishi mushrooms, and ribose supplements for 12 hours before collection.
GPL-SNP1000 Test: Saliva:  Cheek swab   - or -    Blood:  2 mL of whole blood


"After identifying a low serotonin level on an Organic Acid Test (a hallmark functional medicine test), A severely depressed and suicidal man began taking 100mg of 5htp twice a day (5htp is the direct precursor to serotonin). After 2 days, he said his depression virtually disappeared and he was no longer suicidal. I love when I can identify a biochemical glitch and someone can have such a profound emotional turn around."

–Dr. Josh Friedman of Integrative Psychotherapy of Omaha

"The Organic Acids Test has brought new meaning to my specialized practice in women's health. This test has allowed me to investigate more deeply the chronic issues that contribute to female related conditions like PMS, uterine fibroids, endometriosis, chronic UTIs and vulvodynia. I have had a great deal of success in treating complicated PMS cases that didn't respond to typical female hormone balancing therapies. Since utilizing the OAT, it has provided me with a variety of information to determine the underlying cause of female related issues. Some of the markers that I find valuable for women's health issues are the bacterial and yeast metabolites, oxalates, B vitamins, vitamin C, and pyroglutamic acid. I have been so impressed by this test that I can't help but want to spread the word about its uses for various women's health conditions!"

–Jennifer, N.D.